Localization of the xyloglucan in cell walls in a suspension culture of tobacco by rapid-freezing and deep-etching techniques coupled with immunogold labelling
1998
Suzuki, K. (Kyoto Univ., Uji (Japan). Wood Research Inst.) | Baba, K. | Itoh, T. | Sone, Y.
Localization of xyloglucan in cell walls regenerated from tobacco protoplasts (Nicotiana tabacum L.; cv. BY-2) is visualized by rapid-freezing and deep-etching (RFDE) electron microscopy coupled with immunogold electron microscopy. Xyloglucan was already deposited in the cell wall 3 h after culture initiation. Xyloglucan was mainly localized along microfibrils with a lesser amount in intersections between two crossed microfibrils in 120-hour-old cells. These data support the previous hypothesis of Keegstra et al. (1973) that propose an interconnection between xyloglucan and cellulose
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