Prolongation of embryonic diapause by repeated injection of synthetic diapause hormone into pupae of non-diapause egg producer of the silkworm, Bombyx mori
1999
Furusawa, T. (Kyoto Univ. of Industrial Arts and Textile Fibers (Japan). Faculty of Textile Science) | Ueda, N. | Nakamura, N. | Sakano, D. | Kotani, E. | Sugimura, Y.
The injection of synthetic diapause hormone (BomDH-1 (19-Trp); DH) into pupae of non-diapause producer (polyvoltine race) transformed non-diapause eggs into diapause ones (DH-induced diapause eggs). Single injection of the DH (50 to 200 pmol/pupa) into 3.5-day-old pupae produced diapause eggs of 18-27 % per total laid eggs. The DH-induced diapause eggs did not hatch for more than 95 days after oviposition, although non-induced diapause eggs hatches for about 10 days after oviposition. The DH-induced diapause eggs were chilled at 5 degree C from day 15 after oviposition, and thereafter transferred to 25 degree C at various time and incubated for 14 days to estimate hatchability. They resumed embryogenesis but 10 to 50 % of them died at the stage of body pigmentation. However, repeated injections of DH (100 pmol/pupa) elevated the percentage of diapause eggs when compared to single injection. Sorbitol content was about 2 times higher in the eggs induced by the repeated injections (3 or 4 shots) of the DH than in those induced by single injection of the DH. The sorbitol in the former eggs decreased later than that in the latter ones during the chilling at 5 degree C. The decrease in sorbitol content was accompanied with the elevation of NAD-sorbitol dehy-drogenase and of the hatchability. Thus, the repeated injections of the synthetic DH in the pupae of polyvoltine race caused diapause status and the sorbitol metabolism in the DH-induced diapause eggs as well as natural di
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