Extractive acetone-butanol-ethanol fermentation using methylated crude palm oil as extractant in batch culture of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)
1999
Ishizaki, A. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Michiwaki, S. | Crabbe, E. | Kobayashi, G. | Sonomoto, K. | Yoshino, S.
The possibility of employing methylated crude palm oil (CPOE) as an extraction solvent to reduce endproduct inhibition and to enhance solvent productivity in acetone-butanol-ethanol (ABE) fermentation was evaluated using oleyl alcohol as the standard butanol extractant. Fermentation was carried out at an initial glucose concentration of 90 g/l. CPOE did not inhibit the growth of the farmentative organism. Without solvent extraction, butanol production ceased after 30 h at a concentration of 15.4 g/l limiting cell growth to 3.98 g/l and glucose consumption to 62%. Applying CPOE as the extraction solvent, about 47% of the total butanol produced was extracted, glucose consumption was increased to 83% and relatively high glucose consumption rates and solvent productivities were obtained. Butanol production increased to 20.9 g/l; total ABE solvents and yield also increased from 21.2 g/l and 3S% (in conventional fermentation) to 29.S g/l and 40.4%, respectively
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