Inactivation of Bombyx mori nucleopolyhedrovirus (BmNPV) with calcium hydroxide solution
1999
Zhou, C.Q. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science) | Nakagaki, M. | Takizawa, K. | Tsuda, H. | Kajiura, Z. | Takei, R. | Iwashita, Y.
When the nuclear polyhedra were exposed to calcium hydroxide solution, polyhedrin and virus structural proteins were degraded into proteins with the lower molecular weight. The process of virion dissolution in the calcium hydroxide solution was observed by the electron microscope. At first, the uncleocapsids protruded from an opening on the viral envelopes. The protruded capsids were dissolved and then the remaining envelopes were dissolved. Calcium hydroxide solution degraded the genomic DNA of nucleopolyhedrovirus (BmNPV) into fragmented DNAs with the lower molecular weight. In this paper, we have demonstrated that in activation of BmNPV with calcium hydroxide solution results from not only the blocking of viral attachment on and its penetration into cells but also the degrading of the infectious. virus DNA
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