Isolation and genetic determination of a Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase
1999
Hirose, N. (Osaka Prefectural Univ., Sakai (Japan). Coll. of Agriculture) | Fujii, M. | Kishida, M. | Kawasaki, H. | Sakai, T.
A Saccharomyces cerevisiae mutant that produces an endo-polygalacturonase (PGase) was isolated. The PGase gene was revealed to be located on chromosome X in both the mutant and its parental strain. The 5'-upstream region of the PGase gene in the mutant was entirely identical with that of its parent. Crossing of the mutant with a PGase-negative strain showed that the phenotype of PGase production was recessive. These results suggest that the mutant is rendered defective in a transacting factor that normally represses the expression of the PGase gene
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