Changes in protein synthesis and cellular morphology in the fat body of the silkworm, Bombyx mori during larval-pupal metamorphosis
1998
Xu, Y.S. (Utsunomiya Univ. (Japan). Coll. of Agriculture) | Kanke, E. | Kawasaki, H.
Protein synthesis in the fat body was studied in in vitro tissue culture system with 35S-methionine and two-dimensional gel electrophoresis system during last larval instar of Bombyx mori. Number of polypeptide spots and their rate of synthesis increased rapidly in accordance with the development and peaked at 96 hour after the fourth larval ecdysis. Then peptide synthesis decrease resulting in few numbers of spots around the pupation. Rate of polypeptide synthesis was high when the number and size of nucleoli were large. Seven species of newly synthesized polypeptides were detected from wandering stage to the cessation of spinning. During the spinning stage the boundary between cell nuclei and cytoplasm became obscure and protein granules appeared in the cytoplasm. It is suggested that thee 7 polypeptides are synthesized stage specifically from wandering to the cessation of spinning and that they have a correlation with the drastic change of fat body morphogenesis
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