Penicillin and D-alanyl-D-alanine accelerate spore formation of Myxococcus xanthus subcultured cells
1998
Kimura, Y. (Kagawa Univ., Miki (Japan). Faculty of Agriculture) | Ishida, T. | Ujibe, A. | Sato, M.
In repetitive subcultures of Myxococcus xanthus cells, addition of penicillin to the developmental medium accelerated the appearance of the fruiting bodies and spore formation. During development of M. xanthus, DD-carboxypeptidase, which functions in the regulation of the level of cross-linkage, was also induced by penicillin. When D-alanyl-D-alanine, the structure of which is similar to the beta-lactam structure of penicillin, was added to the developmental medium, 0.3 to 0.6 mM D-alanyI-D-alanine accelerated spore formation strongly. The activity of D-alanyl-D-alanine ligase, which synthesizes D-alanyI-D-alanine from D-alanine, increased gradually with maximum activity observed at the initial stage of sporulation. These results raise the hypothesis that M. xanthus produces and releases D-alanyl-D-alanine during development, and the released D-alanyl-D-alanine may function as an inducer of sporulation
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