Effects of mating types of reference family and degree of polymorphism on constructing genetic linkage map
1998
Satoh, M. (National Inst. of Animal Industry, Kukizaki, Ibaraki (Japan)) | Furukawa, T.
Effects of mating types of reference family, and number of marker alleles and the frequency were investigated using Monte Carlo computer simulation. The genetic linkage map was constructed using a reference population of intercross (F2) or backcross comprising of 24 full sib families and 192 progenies derived from a cross between 12 sires and 24 dams. F1 animals were produced by crossing three grandsires from a different breed with six grandams from another breed. Five types of reference populations were used to investigate the effect of mating types: F2, four types of backcross between F1 sires and purebred dams in male or female line, and between F1 dams and purebred sires in male or female line. Each dam produced eight progenies. A chromosomal map length of 1.2M (Morgans), different number of alleles and their frequencies were assumed. The considered average intervals between markers were 0.05, 0.10, 0.15 or 0.20 M with standard deviation of 0.0, 0.01, 0.02 or 0.03 M, respectively. The distance between markers and lod scores were estimated using genotypic data from a reference population. F2 had a higher lod score than backcross, hence F2 was preferred to backcross in the construction of linkage map. Efficiency of linkage analysis in the F2 increased consistently, but the rate declined steadily as the number of alleles increased. The efficiency of linkage analysis did not depend on marker spacing
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