Identification of Ralstonia solanacearum isolated from wilted tobacco [Nicotiana tabacum] plant by fatty acid profiles and PCR-RFLP analysis
1999
Khan, A.A. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Furuya, N. | Matsumoto, M. | Matsuyama, N.
Identification of Ralstonia solanacearum, isolated from wilted tobacco plant was conducted by fatty acid profiles and PCR-RFLP analysis. The tobacco strain showed identical patterns of fatty acid composition with Ralstonia solanacearum and R. pickettii, and it was distinctly different from Burkholderia gladioli pv. gladioli, B. cepacia and B. caryophylli. Palmitoleic acid (16:1 cis 9) content was much higher in tobacco strain, R. solanacearum and R. pickettii. On the other hand, an unidentified (unidentified-6) fatty acid was detected (9.55 to 11.98%) in B. gladioli pv. gladioli, B. cepacia and B. caryophylli, and it was absent in tobacco strain, R. solanacearum and R. pickettii. The tobacco strain showed identical PCR-RFLP profiles (Msp1 digestion) with R. solanacearum and R. pickettii. The PCR-RFLP profiles with HaeIII digestion further differentiated the tobacco strain and R. solanacearum from R. pickettii. Both fatty acid profiles and PCR-RFLP analysis suggested that tobacco strain is R. solanacearum. The physiological and biochemical tests also confirmed this conclusion
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