Characteristics of a mutant of trehalose-producing Micrococcus luteus and optimization of production conditions
1999
Song, H.S. | Hwang, K.C. | Bang, W.G. (Korea University, Seoul (Korea Republic). Department of Agricultural Chemistry)
For the production of trehalose, microganisms cpable of producing trehalose extracellularly were screened from the stock cultures in our laboratory. Among them, Micrococcus luteus IFO 12708 showed the highest productivity of trehalose. For the increase of productivity, the mutant strain HS-208 having higher trehalose production was selected with NTG(N-methyl-N'-nitro-N-nitrosoguanidine) mutagenesis, which led to the decrease of the specific activity of trehalose phosphorylase(3.2-fold) as compared to the wild strain. The optimum condition for the trehalose production was established as follow:20g/l of glucose and 6 g/l of tryptone were used as a sole carbon source and nitrogen source, respectively,and cultivations were carried out at 30 degrees centigrade and pH 6.0. After 20 hrs cultivation, addition of 20 unit/ml penicillin G led to the higher conversion yield of trehalose. Unider the optimum condition, 6.547 g/l trehalose was produced with conversion yield of 32.7%.
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