Genetic analysis of sucrose-accumulating ability in Lycopersicon peruvianum
1999
Egashira, H. (Yamagata univ., Tsuruoka (Japan). Faculty of Agriculture) | Takahashi, S. | Doi, H. | Nishizawa, T. | Escalante, A. | Takashina, T. | Imanishi, S.
The present study was carried out to analyze the sucrose-accumulating ability of the wild accession, L. peruvianum (LPV) var. humifusum LA2153 (LA2153). The mode of inheritance of the PCR band patterns generated with the PCR primers AIT-1 and AIT-2, and the relationship between the PCR band patterns (wild, heterozygous and cultivar types) and their sugar contents, were investigated, using the progeny of interspecific crosses of L. esculentum (LEL) with LA2153. The mode of inheritance of the PCR band pattern revealed one-gene segregation in all of the tested generations. Heterozygous and cultivar types accumulated hexoses, glucose and fructose, but only a small amount of sucrose. On the contrary, wild types accumulated more sucrose than glucose and fructose. From the results of the above experiments and a linkage analysis between the acid invertase gene and the RFLP marker TG102, it was concluded that the sucrose-accumulating trait is controlled primarily by a recessive acid invertase gene closely linked to TG102 on chromosome 3, and that these PCR primers are useful for selecting sucrose-accumulators from the progeny of the cross combination of LEL and LA2153
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