Development of automatic immunosensor system for quantification of allergens
1999
Higuchi, M. (Kyushu Univ., Fukuoka (Japan)) | Kuwano, T. | Matsumoto, K.
A glassy carbon electrode, anodized for a short time at about 2.0 V vs. Pt, is a very convenient regenerable solid phase for the successive steps of an automated enzyme-linked immunosorbent assay. The anodized surface absorbs allergen and the blocking proteins in a similar manner to classical polystyrene solid phase; the detection of the immobilized alkaline phosphatase used as a label can be achieved from an enzymatic process occurring; and the protein stacking, accumulated during the assay, can be eliminated by a simple re-anodization at the end of the cycle, allowing the adsorption of a new allergen. Using peroxidase or alkaline phosphatase at labels, various allergens such as ovalubumin, ovomucoid, and beta-lactoglobulin were assayed
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