Comparison between the sulphuric-peroxide digestion method and the alternative procedures for plant analysis
1994
Sumittra Puvarodom | Nutchari Bunplaeng (King Mongkut's Institute of Technology Ladkrabang, Bangkok (Thailand). Faculty of Agricultural Technology)
K, Ca, Mg, Na, Fe, Mn and Zn of 10 plant materials obtained by sulphuric-peroxide digestion were compared with those determined by acid mixture (HNO3-H2SO4-HClO4) and dry asing procedures. Difference between the methods were not consistent between elements or tissue types but dry ashing generally yielded higher Ca than did sulphuric-peroxide and acid mixture whereas K was slightly lower than the two wet digestion procedures. Mg concentration obtained by sulphuric-peroxide and acid mixture was not significantly difference except for one sample. In contrast, only 7 out of 10 tissue Na content estimated by acid mixture and dry ashing was in good agreement. For micronutrient content in plant tissue, it was found that 8 out 10 Zn and 6 out of 10 Mn values from sulphuric-peroxide and acid mixture were similar. On the other hand, difference in Fe content between digestion methods were highly inconsistent. The results indicated that the two wet oxidation methods were in better agreement when compared to dry ashing. Thus, each laboratory should has its standard procedure for plant tissue determination.
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