In vitro culture of agarwood trees (Aquilaria spp.)
1995
Pimol Tiengtum
In vitro culture of various parts of Aquilaria spp. were studied at tissue culture laboratory, Department of Horticulture, Kasetsart University. Shoot tips and lateral buds from 2 species of agarwood trees (Aquilaria crassna and A. malaccensis) were cultured on Woody Plant Medium (WPM) and modified MS medium with half-strength of nitrate supplemented with BA, 2iP and kinetin at the concentrations rate 0, 0.25, 0.5, 1, 2 and 4 mg/l. Both media supplemented with cytokinin promoted growth and shoot proliferation. BA was the most effective for stimulating shoot multiplication, followed by kinetin. 2iP had no effect on shoot multiplication. Relatively low levels of BA (0.25, 0.5 mg/l) were effective in stimulating the multiplication and elongation of shoot tips and lateral buds. BA at high levels (1-4 mg/l) produced more number of shoots that did not elongate. Callus obtained by culturing young leaf discs on MS medium containing 0.5, 1, 1.5 and 2 mg/l 2,4-D and 2, 3 mg/l BA. But callus did not regenerate. Shoots of A. crassna produced roots on WPM medium with or without auxin. Both NAA and IBA were tested in the rooting experiments, IBA more effective in stimulate rooting. The highest rooting percentage was 65 percent in medium containing 0.5 mg/l IBA. Shoots of A. malaccensis failed to root. The survival rate of A. crassna plantlets was 90 percent when transplanted in nursery.
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