Copper-resistant bacteria from industrial effluents and their role in remediation of heavy metals in wastewater
2002
Shakoori, A.R. | Muneer, B. (University of the Punjab, Lahore (Pakistan). Cell and Molecular Biology Dept.)
Six copper-resistant bacterial strains were isolated from wastewater of tanneries of Kasur and Rohi Nala in Pakistan. Two strains tolerated copper at 380 mg/L, four up to 400 mg/L. Three strains were identified as members of the genus Salmonella; one strain was identified as Streptococcus pyrogenes, one as Vagococcus fluvialis and the last was identified as Escherichia coli. All bacterial isolates showed resistance against Ag+ (280-350 mg/L), Co2+ (200-420), CrVI (280-400), Cd2+ (250-350), Hg2+ (110-200), Mn2+ (300-380), Pb2+ (300-400), Sn2+ (480-520) and Zn2+ (300-450). The isolates were cured of plasmids with ethidium bromide (estimated efficiency: 60-90 %). A reference strain of E. coli was transformed with the plasmids of the bacterial isolates which grew in Luria-Bertani medium containing 100 mg/L of Cu2+. The capability to adsorb and afterwards accumulate Cu2+ inside their cells was assayed by atomic absorption spectrophotometer; all bacterial cells had the ability to adsorb 50-80 % of the Cu2+ and accumulate 30-45 % of Cu2+ inside them after 1 d of incubation.
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