Performance of Bt IR64 rice plants resistant against yellow stem borer in their advance generations
2001
Maiti, M.K. (IIT-BREF Biotek, Indian Inst. of Technology, Kharagpore-721302 (India)) Nayak, P. | Basu, A. | Dandapat, A. | Mandal, C. | Ghosh, D. | Basu, D. | Das, S. | Sen, S.K.
The Yellow Stem Borer (YSB; Scirpophaga incertulas), a major lepidopteran insect pest of rice, causes huge loss of grain production in the farmer's field. Development of Bt (Bacillus thuringiensis) rice plants to protect against such insect damage in the field is in the making in different laboratories, following the documented past successes achieved in corn, cotton, potato and other crop plants. On the same line of progress, a number of Bt transformant lines was generated in IR64, an elite indica cultivar, with a reconstructed Cry1Ab gene expressing the toxin peptide in plant. A few sselected lines were carried through to their fifth generation and the stability of the expression of the transgene judged. Observations indicated the following: 1. that resistance against insect attack in outdoor condition is significantly different from that of laboratory condition; 2. the inheritance of the transgene in certain cases did not entirely tally with the Mendelian inheritance pattern; 3. seasonal variation of rice cultivation does influence the capacity of resistance of the Bt lines against insect infestation; 4) supplementary application of chemical insecticides in winter cultivation helped protection of the transgenic crop; 5. selection of a few transgenic lines showing stable expression and protection against insect attack was possible
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