Effect of salinity on growth of Eucalyptus seedlings in vitro
1998
Suriyan Cha-um | Chalermpol Kirdmanee | Kriengkrai Masaleeyanon | Ruanrudee Vanatsakul (National Science and Technology Development Agency, Bangkok (Thailand). National Center for Genetic Engineering and Biotechnology)
Objective of this investigation was to discover the in vitro selection procedures to obtain salt tolerant clones. Eucalyptus seeds (Eucalyptus camaldulensis Denn.) were sterilized with 1.6 percent sodium hypochlorite (w/v) for 30 minutes and rinsed three times with sterile distilled water. The seeds were cultured on Murashige & Skoog (MS) agar medium. After culturing for 8 weeks, seedlings were treated with 0, 2, 4 and 6 percent sodium chloride (NaCl). Survival percentage, fresh weight, dry weight, leaf area, and chlorophyll content were measured after treatment for 4 weeks. The fresh weight and leaf area significantly decreased with increasing salinity. Two percentage of NaCl caused approximate 50 percent reduction of the fresh weight and leaf area. Increasing level of NaCl were decreased the chlorophyll content and leaf succulence. This result of decreasing the chlorophyll content and leaf succulence should be a caused of low survival percentage.
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