Effect of accelerated ageing on thr content and composition of polyphenolic complex of wheat (Triticum aestivum) grains
2003
Lachman, J. | Dudjak, J. | Orsak, M. | Pivec, V. (Ceska Zemedelska Univ., Prague-Suchdol (Czech Republic))
The accelerated ageing test (AAT), i.e. the influence of higher temperature and humidity, on the content and composition of phenolic compounds in the grains of five cultivars of wheat (Triticum aestivum) was studied in the years 1998 and 1999. Total polyphenols were determined spectrophotometrically by Folin-Ciocalteau`s phenolic reagent and free phenolic acids by an HPLC method in control samples and after deterioration treatment (AAT) in the Ebi, Estica, Nela, Samanta and Sarka cultivars. Statistical significance of deterioration treatment, cultivar, cultivation site, and harvest year was proved. Content of total polyphenols increased during deterioration and levels of the individual free phenolic acid changed depending on their structures.
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