Extension of the spectra of plant products for the diet in coeliac disease
2003
Petr, J. | Capouchova, I. | Famera, O. (Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Rostlinne Produkce) | Michalik, I. | Tlaskalova, H. | Urminska, D. | Tuckova, L. | Knoblochova, H.
Concentrations of the total and protein nitrogen, the composition of protein fractions, the electrophoretic composition of reserve gluten and prolamine proteins were determined in different crops. Gliadin concentration, quantified using ELISA test, were below 10 mg per 100 g in the following species: Amaranthus hypochondriacus and A. cruentus, Chenopodium quinoa, Sorghum bicolor and S. saccharatum, Panicum miliaceum, Setaria italica ssp. maxima, Digitaria sanguinalis, and Fagopyrum esculentum. These species can be considered as suitable for the diet in coeliac disease. Below-limit values were found in triticale (Triticosecale) and some oats varieties. The analysed samples differred by crude protein concentration and fraction structures of the protein complex. Electrophoretic analysis PAGE of prolamine proteins or SDS-PAGE ISTA, developed for gluten proteins, confirmed the results of immunological tests on the suitability of quinoa, grain sorghum, sweet sorghum, buckwheat, amaranth, broadrood, millet and foxtail millet for the diet in coeliac disease. These species did not contain prolamins or alpha-prolamins concentration was negligible in the given samples. The tested species of wheat, triticale, and oats species were manifested as substandard or unhealthy for the diet.
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