Involvement of xylem sap zeatin-O-glucoside in cucumber shoot greening
2002
Kato, C. ((University of Tsukuba (Japon). Institute of Biological Sciences)) | Kato, H. | Asami, T. | Yoshida, S. | Noda, H. | Kamada, H. | Satoh, S.
Francés. A fraction of xylem sap collected from squash (Cucurbita maxima) root and separated with gel filtration showed strong promoting activity in a greening bioassay using etiolated cucumber cotyledons. This activity decreased with waterlogging of the root. The promoting factor was purified with sequential gel-permeation, and reverse-phase and normal-phase column chromatographies. Zeatin-O-glucoside (ZOG) was identified as the promoting factor by monitoring liquid chromatography tandem mass spectrometry. Although the glycosyl conjugate of zeatin was thought to be inactive, ZOG had a promoting activity that was 100 times higher than those of zeatin and zeatin riboside between concentrations of 10(-9) to 10(-4) M in the greening bioassay. These results suggest that in some developmental stages, the conjugation of cytokinins with a sugar moiety, such as glucose, might be a key factor in the control of shoot greening by roots
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