Alternative media for large-scale culture of Tetrahymena sp.
1998
Matias, R.R. | Natividad, F.F. | Dimamay, M.P.S. (Philippines Univ. Diliman, Diliman, Quezon City (Philippines). Molecular Biology and Biotechnology Program)
Alternative media using skimmed milk and corn steep liquor supplemented with yeast extract and glucose were used in mass cultivation of Tetrahymena sp. Corn steep liquor, an industrial waste of corn chips production, and skimmed milk, are sources of protein in culture media. Cultivation of cells was optimized by varying the concentrations of media components particularly skimmed milk, corn steep liquor, and glucose, and by varying culture conditions. Cultivation was done in a 2.5 L bioreactor supplied with a digital control unit which regulates culture conditions such as pH, dissolved oxygen, and temperature. Under these conditions, faster doubling time (4hrs) and higher amount (9 g/L) of biomass were obtained compared with the standard protease peptone media which exhibits 10 hrs generation time and yields 2 g/L biomass. The use of corn steep liquor as an alternative culture medium maybe a possible way of recycling industrial waste
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