Isolation and identification of Borrelia burgdorferi s. l. strains from ticks Ixodes ricinus
2001
Borko, C. (Ljubljana Univ. (Slovenia). Veterinary Fac.) | Ruzic-Sabljic, E. (Ljubljana Univ. (Slovenia). Medical Fac.)
The autors isolated and identificated Borrelia garinii spirochetes from ticks of Ixodes ricinus species, gathered in different parts of Slovenia and which were then subsequently used to invade laboratory mice (Mus musculus). Isolation was done in BSKH medium containing 6 % of rabbit serum. Two isolates were obtained from ticks (20 %) and one indirectly from mouse (11 %). In all cases the Borrelia garinii was isolated. In the present study the method of pulsed field gel electrophoresis (PFGE) after restriction of genomic DNA with Mlu I was applied for identification of species. PFGE was also performed for the presentation of plasmide profile.
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