Identification of Rhizoctonia spp. using polyclonal antiserum in EBIA
2002
Vico, I. (Poljoprivredni fakultet, Beograd - Zemun (Serbia and Montenegro)) | Krstic, B. | Tosic, M.
A polyclonal antiserum (As-1) raised against total mycelial antigens of binucleate Rhizoctonia AG-I was used in EBIA in order to determine antigenic properties and serological relationship of some binucleate and multinucleate Rhizoctonia spp. After separation by electrophoresis and based on the reactions with antiserum As-1, it has been established that a total of 18 protein fractions show antigenic properties (14.4-94 kDa). The number and patterns of protein bands differed in binucleate and multinucleate Rhizoctonia isolates and their anastomosis groups (AG). Protein fraction designated as number 8 was common to most of the isolates, but the profiles of the remaining bands pointed out serological differences. The results show that the use of polyclonal antiserums can be helpful in detection of different Rhizoctonia spp., but, what is more important, when a polyclonal antiserum is used in EBIA, it is possible to differentiate anastomosis groups of binucleate or multinucleate Rhizoctonia spp.
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