Changes in the content of modified nucleotides in wheat rRNA during greening
2003
Paradi, I. | Rudnoy, S. | Bratek, Z. | Kovacs, G. | Racz, I. | Lasztity, D. (Eoetvoes Lorand Univ., Budapest (Hungary)) | Paldi, E.
Changes in the state of modification of 5.8S and 18S rRNAs were examined during the greening of etiolated wheat seedlings. Three types of minor nucleotides, O2'-methyladenosine, O2'-methylguanosine and pseudouridine were found in the 5.8S rRNA of green wheat leaves, none of which was detected in etiolated wheat. The minor nucleotides appeared in the 5.8S rRNA only after 48 h irradiation. The sequences of 5.8S rDNA, ITS1, ITS2 and 18S rDNA were also determined and the presence of the hypermodified nucleotide 1-methyl-3-(alpha-amino-alpha-carboxypropyl)-pseudouridine was detected in green wheat 18S rRNA. This minor component was not demonstrable in etiolated wheat 18S rRNA, but appeared after irradiation for 48 h.
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