Thidiazuron induced multiple shoot induction and plant regeneration from cotyledonary explants of mulberry
2003
Thomas T.D. (St. Thomas College, Pala (India). Post Graduate and Research Dept. of Botany)
A rapid micropropagation protocol through induced multiple shoots from the cotyledonary explant of mulberry is described. The highest number of shoots (20.3) was obtained when explants from 14-d-old embryos were cultured on Murashige and Skoog (MS) medium supplemented with 7 microM thidiazuron for 45 d. Of the three cultivars used, cv. S-36 was the best followed by cv. K-2 and S-1. The shoots were transferred to MS medium supplemented with 5 microM 6-benzylaminopurine for elongation. The elongated shoots were rooted on half strength MS medium containing 1-7 microM indole 3-butyric acid or 1-naphthalene acetic acid. The rooted plants were transplanted to soil with 90 % success. The emerged shoot primordia probably initiated from the pre-existing meristems since the shoot bud show definite vascular connection to the major vascular tissue.
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