Partial Purification and properties of chitinases from skin and tissue of Zingiber officinale rhizome
2003
Bodhini, D. | Palani, P. | Vidyalakshmi, A. | Balasubramanian, R.(University of Madras, Chennai (India))
Colorimetric and enzyme activity staining techniques on polyacrylamide gel indicated the presence of chitinases in the skin (outermost layer) and the tissue of Zingiber officinale rhizome. The chitinase activity was significantly higher in the skin than the tissue and the vice-versa was true for the protein content. Chitinases were partially purified using gel filtration and ion exchange chromatographic techniques from skin and tissue. The partially purified chitinases of skin and tissue showed an apparent molecular masses of 29 and 34 kDa, respectively. The enzymes showed their activity at an optimum pH of 5.0. The optimum temperature of skin chitinase was 40ø C while that of the tissue was 40ø C as well as 50ø C. Both the enzymes hydrolysed colloidal chitin better than the other substrates. Inhibition of conidial germination of the test fungi by the enzymes proved their antifungal role.
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