RAPD, GISH and FISH Analysis for Detection of Rye Chromatin Introgressed into Wheat
2003
Lee, J.O. | Shin, D.I. | Chung, I.K. (Kyungbuk, Republic, of Korea) | Ko, J.M. | Han, S.I. | Hyun, J.N. (Milyang, Republic of Korea) | Park, S.K (Daegu, Republic of Korea) | Do, G.S. (Daegu, Republic of K
Rye (Secale cereale L.) is known as an important source of useful genes for the improvement of resistance go rust, powdery mildew and tolerance to drought and cold on wheat (Triticum aestivum L.). We describe the identification and utilization of rye genome-specific DNA markers, OPH20-1494 bp and OPC10-1012, by RAPD analysis. These markers were used to detect rye chromatin among467 BC1F6 lines derived from the backcross of the F1 hybuid of wheat cv. Paldanghomill. Thirty-two lines out of 467 BC1F6 population possessed the part of rye genome. Moreover GISH and FISH analysis confirmed the presence of uye chromosome fragment on wheat background in these lines. Therefore these uye-specific markers can be used effectively in the breeding program for introgression of rye chromatin into wheat.
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