Induction of in vitro shoot proliferation and rooting of axillary buds of pineapple cv queen (Ananas comosus (l.) Merr.)
2004
De Juras, R.J.C. | Aspuria, E.T. | Espino, R.R.C. (Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Horticulture)
The responses of axillary buds of pineapple CV. Queen to plant growth hormones and coconut water supplementation in Murashige and Skoog's basal medium (MS) were evaluated. Maximum number of multiple shoots were obtained from the explant grown in MS + 2.0 ppm kinetin (Ki) + 2.0 ppm naphthalene acetic acid (NAA) + 2.0 ppm indole-3-butyric acid (IBA) with an average of 6 shoots per culture. All in vitro proliferated shoots produced roots in rooting media supplemented with different concentrations (0, 0.1, 0.5, 1.0 and 2.0 ppm) of NAA and indole-3-acetic acid (IAA) added singly or in combination. The number of days to root formation and the number of roots formed were directly proportional to the concentration of the NAA when added in the culture medium. On the other hand, root elongation was inversely proportional to the concentration of NAA. The regenerated plantlets were successfully established in plastic pots containing sterilized soil with 95% success
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