Soil microbial community development
2004
Malabayabas, M.D. | Xuan, T.H. | Nazar, J.S. | Corales, R.G. (Philippine Rice Research Inst., Maligaya, Munoz, Nueva Ecija (Philippines). Central Experiment Station)
Six treatments were employed, and LCC-based [leaf color chart] N rate was 30 kg N/ha while all LCC-based treatments were applied with N at 27 and 42 DAS. Fresh soil samples taken periodically were analyzed through chloroform fremigation for biomass N, chlorophyll A extraction for phototrophic biomass, and Electron Transport System (ETS) for microbial activity. Air-dried soil samples were used in the isolation of soil microorganisms. Initial identification was done by gram-stain technique or by Mac Conkey agar to identify gram-positive or negative organisms. Final identification was done using the BIOLOG for gram-negative isolates, and BBL Crystal for the gram-positive. Biomass N with rice compost + LCC-based N application and EM organic was significant probably due to the N from chemical fertilizer and the high carbon content of rice straw. Chicken manure combined with N application and EM organic had higher chlorophyll A than the other treatments due to the faster release of N. Higher chlorophyll A in EM organic can be attributed to the presence of photosynthetic bacteria. ETS analysis showed that all treatments applied with organic fertilizer had significantly higher microbial activity than those fertilized with chemical fertilizer. The presence of carbon source or inoculation probably increased microbial activity. Ten bacterial species were isolated from the field setup, most of which belong to the Bacillus group: Treatments with EM proved to have higher number and species of beneficial microorganisms. Ecological farming in the community was promoted through trainings, seminars, demonstrations, and publications
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