Renal clearance of diclofenac sodium in female volunteers after oral administration

Renal clearance of endogenous creatinine and diclofenac, sodium was investigated in nine healthy human female volunteers, after oral dose of 50 mg tablet. Blood and urine samples were intervals after drug administration and analyzed for creatinine, and diclofenac sodium collected at pre-determined time concentration by high performance liquid chromatography (HPLC) method. Their renal clearance was determined on the rate of urine flow (diuresis) was 0.036 + 0.010 ml/min/kg, pH of blood was 7.56 plus minus 0.01 and of urine was 5.88 plus minus 0.147. Concentration of creatinine in plasma and urine was 9.468 plus minus 0.430 and 558.91 plus minus 91.38 mug/mL, respectively. The mean plus minus SE value of dicolfenac sodium in plasma and urine was 0.505 plus minus 0.057 g/mL and 29.97 plus minus 3.55 mug/mL, respectively. Renal clearance of creatinine and diclofenac 1.236 plus minus 0.226 and 2.826 plus minus 0.755 mL/min/kg body respectively. The clearance ratio was 2.491 plus minus 0.285. The average plus minus SE 0.036 plus minus 0.010 mL/min/kg, pH sodium was weighted, respectively. The clearance ratio was 2.491 plus-minus 0.285. The studies indicate that the renal clearance of endogenous creatinine in female volunteers was less than the values given in literature. A highly significant positive correlation between diuresis and renal clearance of diclofenac sodium is indicative of renal tubular back diffusion or re- absorption. There is a non-significant positive correlation between urine pH and diclofenac sodium clearance. There is significant positive relation between diuresis and clearance ratio and also non-significant positive correlation between pH or urine and clearance ratio. There is significant negative correlation between plasma concentration and clearance ratio and also significant negative correlation between plasma concentration and drug clearance as shown by results and graphs that beside Glomerular filtration, is concluded mechanisms of back diffusion and active tabular secretion was also involved in diclofenac sodium excretion.