Analysis of blood of human beings (females) for acetylsalicylic acid

The concentrations of acetylsalicylic acid as free salicylic acid in the plasma of twelve healthy female volunteers were determined by calorimetric analysis following oral administration of 2 x 300 mg tablets. The acetylsalicylic acid molecule was found to absorb rapidly and the concentration was detected even after 15 minutes of drug intake by the volunteers. The plasma time data was used for the determination of absorption and disposition kinetic parameters of the drug by t compartment open model. After oral administration of acetylsalicylic mean + SE values for the absorption kinetic parameters were for time to peak concentration (tmax) with mean plus minus SE 1.87 plus minus 0.05 hour (range 1.65 to 2.22) hours, Cmax 44.54 plus minus 0.46 mg, /L. (range 40.72 to 46.63 mg/L) absorption half life 1.17 plus minus 0.25 hours (range 0.45. to 2.88 hours) and the absorption rate constant (Ka) 0.85 plus minus 0.12 1/h (range 0.241 to 1.525 1/h) and average lag time of the drug was 0.13 plus minus 0.01 h (range 0.096 to 0.158h). The disposition kinetic parameters revealed mean plus minus SE values for area under curve, (AUC) 275:07 plus minus 2.73 h.mg/L and range between 257.1 to 2900 h.mg/L volume of distribution 10.90 plus minus 0.76 L, range (6.79 to 16.62 L), distribution half life 0.75 plus minus 0.07 hours range (0.514 to 1.324 hours) and elimination half life 4.69 plus minus 0.55 hours range (2.68 to 9.65 hours) and the mean residence time (MRT) 7.44 plus minus 0.57 hours range (6.28 to 12.8 hours) and total body clearance TBC 1.68 plus minus 0.06 L/h range (1.19 to 1.92 L/h). The mean maximum concentration of aspirin as free salicylic acid in plasma was 49.66 plus minus 1.33 g/ml at 120 min. The result of this study indicated that bio-kinetic parameters of acetylsalicylic acid in healthy female volunteers are within comparable range. Different indigenous conditions affect the pharmacokinetic parameters on which the rational dose regimens of drug could be based.