Development of enzyme linked immunosorbent assay for seromonitoring against hydropericardium syndrome (ELISA) virus
2003
Manzoor, R. | Kamal, T. | Khawaja, D.A. | Ismail, M.A. | Gill, Z.J. (Veterinary Research Inst., Lahore (Pakistan))
In this study ELISA was standardized for detecting antibodies against Hydropericardium Syndrome Virus. Factors like Antigen, Conjugate and incubation time were studied. Standardization was carried out by checker board titration method. Optimum incubation time for colour development was found to be 15 minutes while 50 mug/ml of antigen gave better results than 100 mug/ml. The conjugate dilution of 1:500 was observed to give satisfactory results as determined by regression analysis. Cut off value was determined by adding 2SDs and 3SDs in the mean optical density (OD) values of negative serum samples. It was found that mean OD+2SD (0.0684) resulted in better diagnostic sensitivity) and diagnostic specificity than mean OD+3SDs (0.0741).
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