Embryo rescue in inter-specific crosses of Lupinus albus and L. mutabilis
2004
Shah, A.H. | Haq, N. (University of Southampton (United Kingdom). Dept. of Civil and Environmental Engineering)
Inter-specific hybridization of Lupinus albus and L. mutabilis was attempted using exogenous plant growth regulators and in vitro embryo/ovule culture. Observations on pod and ovule development in reciprocal crosses revealed that the process of embryo abortion starts between 3rd and 5th day after pollination and is the appropriate stage for embryo rescue through in vitro embryo/ovule culture. Application of plant growth regulators had some positive effects on pod retention but did not stop pod abscission. Hybrid ovules were cultured from both the combinations and some of them developed in vitro into plantlets.
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