Rice panicle response to in vitro and in vivo systems
2002
Zhao, X. | Darvey, N.
This chapter describes a systematic approach to in vitro culture of rice for both young panicles and controlled-environment experimentation on tolerance for various stresses from early microsporogenesis through to pollen development. this experimentation evaluates current progress with panicle culture technology, with special reference to microsporocytes and uninucleate microspores. In this procedute, immature detached rice panicles were cultured in medium, using different combinations of growth regulators, and young panicle development in vitro resulted in well-developed panicles with normal glumes, however, the process was much slower than in vivo. The anthers failed to undergo further development and did not show physical growth in most of the cultures. In addition, further development was not observed in cultures in which panicle size was less than 2 cm in length. Individual florets were also cultured, and they gave a similar response as in panicle culture. Different basic media did not have a significant effect on panicle culture response. Growth regulators such as indole acetic acid (IAA) and benzylaminourine (BAP) enhanced panicle development in vitro, whereas gibberellic acid (GA3) showed negative effects. Liquid medium gave better results than solid medium. Other experiments using a hydroponic system was discussed. This system aimed at producing large numbers of panicles for controlled-environment experiments.
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Este registro bibliográfico ha sido proporcionado por International Rice Research Institute