Using DNA marker to identify groundnut hybrid in groundnut rust resistance research
2004
Surson, S. | Loahasiriwong, S. | Prathepha, P. | Wongkeaw, S. (Khon Kaen Univ., Khon Kaen (Thailand). Faculty of Agriculture. Dept. of Agronomy)
There many important steps in breeding for rust resistant groundnut cultivar e.g. evaluation of resistance levels, and population building. Groundnut is self-pollinated crop, it has a high rate of self-pollination in the breeding program. The use of DNA to classify hybridization would help to make more accurate selection and speed up the progress of work. The population of this study was from crosses of susceptible cultivars (KKU1 and Tainan 9) and resistant cultivar (NC Ac 17090). It was found that in F sub(1), hybrids had many characteristics in between the parents characters. For example, hybrid of Tainan 9xNC Ac 17090 had no difference from Tainan 9 in seed weight per plant, width and length of pod, but pod per plant and pod weight per plant had higher values than those of Tainan 9 (high yield cultivar). However, hybrid of KKU2 x NC Ac 17090 had seed weight per plant, width and length of pod values in between those of parents e.g. lower than KKU 1 but higher than NA Ac 17090. From RAPD technique, 120 primers have been screened. Only one primer (OPO11) showed a difference between NCAc 17090 and susceptible cultivars (KKU1 and Tainan 9) at 1000 base. So, it was introduced as a tool to select F sub(1) hybrid. The results indicated that F sub(1) hybrids were 56.25 and 57.69 percent from crosses of Tainan 9 x NCAc 17090 and KKU1 x NCAc17090 respectively. Results from morphological study confirmed that those plants were from hybridization. Correlation of pustule diameter and number of pustules were significant. Results from F sub(2) indicated that the ratio of susceptible to resistant plants was 15:1 (p0.05). However, only 50 percent of plant with small pustule showed O11 sub(1000) marker.
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