Formation of naphthoquinones in Impatiens balsamina L. cell suspension cultures
2000
Pharkphoom Panichayupakaranant | Pramote Pannueng (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Phamaceutical Science. Dept. of Pharmacognosy and Pharmaceutical Botany)
Formation of naphthoquinones in Impatiens balsamina L. cell suspension cultures was achieved using the leaves of parent plants with high yiled of 2-methoxy-1,4-naphthoquinone (0.19 percent dry weight) as an initiating explant. The cell suspension cultures with red-yellowish color were established in B5 medium supplemented with 0.1 mg/2,4-D and 1.0 mg/l BA. The cell cultures were capable of producing two naphthoquinones, lawsone and an unknown which is more polar than lawsone. A study on the time-course of growth and laswone production in I. balsamina cell culture showed that lawsone was biosynthesized in the late phase of the culture cycle.
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