Preliminary testing of biomass production of Hirsutella thompsonii var. synnematosa
1999
Saowanit Maimala | Angsumarn Chandrapatya | Chiradej Chamswarng | McCoy, C.W. (Department of Agriculture, Bangkok (Thailand). Entomology and Zoology Div. Fruit Trees, Herb and Spice Crops Entomology Research Group)
Hirsutella thompsonii var. synnematosa no. 30 was cultured on 3 different types of media : semi-solid, solid and liquid media. Colonies on Soybean agar produced higher numbers of spores, (9.15*10*[7) spores/ml), where those on malt extract agar provided significantly higher number of colony forming units (CFUs) (4.78*10*[9) CFU/ml). Semi-solid agar was not recommended for mass production of Hirsutella due to its high cost. Ground corn coated with molasses was the best solid medium generating 3.07*10*[7) spores/ml with 2.64*10*[7) CFU/ml. Suitable liquid medium comprised 8 percent unpolished rice, 2 percent molasses and mineral salts. Dried mycelium harvested from liquid medium contained 7.92*10*[6) CFU/g. The fungus failed to produce spores in the broth, but many chlamydospores were observed within the hyphae.
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