Induction mutation of mangosteen by colchicine treatment with shoot bud cultured in vitro
1999
Te-chato, S. | Sujaree, R. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
Cluster of managosteen buds was cultured in medium supplemented with colchicine ranging from 0 to 10,000 mg/l for 2 hours to 30 days in order to induce polyploidy and thus mutation. The result revealed that culturing under the colchicine concentration of 1,500 mg/l for 2 hrs provided a non-significant difference in mean survival of shoots but proved significant in the size of shoot, number of roots, leaf number and leaf areas. In addition, application of 500 mg/l colchicine induced higher chlorophyll a content than other concentrations. Increasing the treatment duration to 10 hours and concentration to 3,000 to 10,000 mg/l reduced the percentage of bud-forming shoots, whereas chlorophyll a and total chlorophyll content increased. Treating buds with colchicine at 500, 750 and 1,000 mg/l for 30 days decreased the average number of shoots, and the percentage of bud-forming shoots. These concentrations promoted elongation of roots but reduced the number of leaves, while producing no significant change in chlorophyll content. When the concentration of colchicine was increased to 3,000, 6,000 and 10,000 mg/l, the percentage of bud-forming shoots fell to 12 percent and developed shoots were stunted, followed by leaf dropping. A number root tip chromosomes could not be distinguished between the colchicine treated and control due to their small size. The number and size of guard cells varied. Treating with 750 and 1,000 mg/l colchicine for 30 days caused an increment in the size and color of guard cells. A study on 4 systems of isozyme revealed that peroxidase and esterase can preliminarily distinguish between treated and non-treated plantlets.
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