Isozymes electrophoresis for varietal verification in some tropical vegetables
1993
Panie Temiesak | Kasemsak Palakorn | Karnda Tantiyavarong | Pornpan Pooprompan (Kasetsart Univ. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex)
Techniques for varietal identification of vegetable crops using seed protein and isozymes electrophoresis were investigated. Several isozymes namely, alcohol dehydrogenase (ADH), peroxidase, esterase, glutamic oxaloacetic transaminase (GOT), phosphoglucomutase (PGM) and malate dehydrogenase (MDH) were analysed to standardize in proper condition of plant ages, plant parts, sample extraction solution, gel concentration and staining solution. It had been found that 12 percent gel was suitable to analyse isozymes and 13.5 percent for protein. Isozymes extracted with phosphate buffer, pH 7.5-8.5 from leaves of 7 day old seedling showed the best expression of banding pattern and distinguish the difference among breeding lines, namely peroxidase in sweet corn, ADH, PGM and esterase in Brassica; esterase in tomato and okra.
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