Callus induction and plant regeneration from barley mature embryos (Hordeum vulgare L.)
2005
Abumhadi, N. | Kamenarova, K. | Todorovska, E. | Dimov, G. | Atanasov, A. | Trifonova, A. | Gecheff, K.
Immature and mature embryos of four Bulgarian winter and spring barley (Hordeum vulgare L.) varieties were cultured in vitro to compare the levels of callus induction and plant regeneration. Immature embryos, 12-16 days after anthesis were aseptically excised and placed with the scutellum down wards on callus induction solid medium based on Murashige and Skoog(MS) mineral salts and supplemented with 2mg/l 2,4-D dichlorophenoxyacetic acid. Mature embryos were aseptically excised from the imbibed seeds and were placed with the scutellum down on callus induction medium based on MS containing 2mg/l 2,4-D. The developed calluses were maintained on MS medium containing 1mg/l 2,4-D and 0.5mg/l BAP 6-Benzylaminopurine. The regenerated plants were maintained on MS free hormones medium.A comparison of the responses of the two types of explants indicated that the mature embryos were more useful explants for plant regeneration, and therefore, being available throughout the year, can be used as an effective explant source in barley studies.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institute of Agricultural Economics