Virulence of Fusarium species to alfalfa seedlings
Krljaja, V.(Institute for Animal Husbandry, Belgrade - Zemun (Serbia and Montenegro)) | Levic, J. | Ivanovic, M. | Tomic, Z.
In in vivo conditions, virulence of 91 isolates of species Fusarium genus (Fusarium oxysporum, Fusarium solani, Fusarium acuminatum, Fusarium equiseti, Fusarium arthrosporioides, Fusarium proliferatum, Fusarium avenaceum, Fusarium semitectum, Fusarium tricinctum, Fusarium sporotrichioides and Fusarium graminearum) towards alfalfa seedlings was investigated. Isolates of investigated species originated from diseased alfalfa plants collected at four locations in Serbia (Serbia and Montenegro) based on symptoms of wilting caused by fusarium and root rotting. Pathogenicity and virulence of investigated isolates of Fusarium spp. were determined by visual evaluation of inoculated seedlings of cultivar K28 in laboratory conditions. All isolated of investigated species had pathogenic effect on alfalfa seedlings, which expressed symptoms such as necrosis of root, moist rotting and 'melting of seedlings'. Colour of necrotic root tissue varied from light brown, brown, lipstick red to explicit black, depending on the Fusarium species. Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates.
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