Evaluation of field methods for determining immunoglobulins in sucking foals
Sedlinska, M.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Klinika Chorob Koni)E-mail:[email protected] | Krejci, J.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) | Vyskocil, M.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Chovu, Plemenitby Zvirat a Genetiky)
The aim of the study was to evaluate quick and easy semiquantitative tests for the detection of serum immunoglobulins in foals. We compared the four most employed methods as follows: zinc sulphate turbidity tests using photometric or visual reading, glutaraldehyde test and refractometric determination of total protein concentration in serum. Radial immunodiffusion test was used as a reference method. The tested methods were correlated with the reference method. Nevertheless, we cannot recommend any of these methods as reliable. On the other hand, the practical usefulness of visual reading of turbidity and refractometry for rapid field identification of hypogammaglobulinaemic foals was confirmed. We prefer the refractometric method (sensitivity: 94.11; specificity: 83.30 and predictive value: 88) for common use because it is quicker and easier than the other methods.
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