The effect of gonadotropin releasing hormone on superovulation in elite swamp buffalo cows (Bubalus bubalis)
2001
Techakumphu, M. (Chulalongkorn Univ., Bangkok (Thailand)) | Sukavong, Y. | Intaramongkol, S. | Intaramongkol, J.
The effect of gonadotropin releasing hormone (CnRH) supplement was investigated in twenty eight FSH-treated buffalo cows. Animals were assigned to three groups; Group 1: GnRH was given at standing heat (n = 9), Group 2: GnRH was given 8 - 12 hr after standing heat (n = 8) and Group 3: Control group with FSH alone (n = 11). The responses (no. of corpora lutea and no. of anovulatory follicles), the number of recovered embryos and transferable embryos among the three group were compared following slaughter of the animals on days 6 to 7 after first mating. The results indicated that the application of GnRH in FSH-treated animals gave no advantage by increasing in the number of ovulations or recovered embryos in all the treatment groups (P > 0.05); 4.33 =+- 3.35 vs 3.88 =+- 4.09 vs 4.5 =+- 2.68 for corpora lutea, and 2.33 =+- 2.24 vs 2.0 =+- 3.20 vs 1.91 =+- 2.74 for recovered embryos respectively. CnRH treatment tended to reduce the number of anovulatory follicles but the finding was not significant; 6.11 =+- 3.3 vs 7.38 =+- 4.84 vs 10.18 =+- 2.74 follilcles (P > 0.05). The supplementation of GnRH at 8-12 hr after standing heat seemed to produce more transferable embryos than those of treated at standing heat or the controls 1.63 =+- 2.77 vs 1.25 =+- 1.67 vs 1.36 =+- 1.69 embryos respectively.
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