The effects of benzyladenine, gibberellin and paclobutrazol on organogenesis in Cymbidium species
2002
Shimasaki, K. (Kochi Univ., Nankoku (Japan). Faculty of Agriculture) | Tsuneisi, J. | Fukumoto, Y.
Addition of paclobutrazol (PAC) to modified MS medium induced prptocorm-like body (PLB) formation from rhizome segment cultures of Cymbidium kanran Makino (terrestrial type). The combination of BA with PAC reduced the number of PLB. Application of BA induced shoot (protocorm-like pseudobulb with short leaves) without root. Single addition of 10 micro M BA had a significant effect on induction and increased the number of shoots. PAC in culture medium inhibited BA-induced leaf formation from rhizome cultures. Application of GA sub3 to PLB cultures of epiphytic type Cymbidium (C. Hiroshima Golden Cup 'Sunny Moon') resulted rhizome formation. GA sub3 treatment at 100 micro M was effective for rhizome formation from PLB cultures. Single addition of 10 micro M PAC was effective for proliferation of PLB from 'Sunny Moon'.
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