Serogrouping and in vitro antimicrobial susceptibility of Salmonella species isolated from Rattus tanegumi Temmick 1844 caught in Los Banos, Laguna, Philippines
Gruezo, C. L. G.(Department of Agriculture, Quezon Memorial Circle, Quezon City 1101 (Philippines). Regional Field Unit 4-B, 4/F)) | Baldrias, L. R. | Sabino, N. G.
Forty-three individuals of Rattus tanezumi Temminck 1844 caught from ricefield, dairy farm, and secondary forest in Mt. Makiling (Los Banos, Laguna, Philippines) were used to isolate Salmonella. A rate of 16% (7/43) of presumptive Salmonella organisms was obtained after selective plating on Xylose Lysine Deoxycholate (XLD) Agar and Bismuta Sulfite Agar (BSA). Biochemical results showed that 14% (6/43) of the isolates could be presumptive Salmonella. These isolates exhibited typical characteristics of Salmonella on Triple Sugar Iron (TSI) agar (red slant, black butt). Lysine Iron Agar (LIA) (violet slant, violet-black butt), Methyl Red (MR; +), Voges Proskauer (VP;-) and urease test (-).On serelogical testing, 4.65% (2/43) was confirmed to be Salmonella belonging to Serogroups E/G, which is also known to consist of strains pathogenic to man. The serologically confirmed isolate were then subjected to in vitro antimicrobial susceptibility testing and were found susceptible to chloramphenicol (30 ug), trimethoprins - sulfamethoxazole (1.25/23.75 ug) and tetracycline (30 ug).
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