Biosynthesis of rice S-poor and S-rich prolamins is regulated by an independent genetic system
2003
Matsusaka, H. | Kumamaru, T. | Ogawa, M. | Satoh, H.
Novel mutants induced by the nitrosomethyl urea (MNU) treatment were isolated and the genetic mechanism and biochemical properties were studied. We isolated 116 prolamin mutants in Taichung 65. These mutants were classified into two groups and subdivided into five types based on the SDS-PAGE band patterns. One group (group A) containing two types was characterized by a remarkable decrease in 13b polypeptide, and the other group (group B) with three types had a significant reduction in 13a polypeptides with a large amount of 13b polypeptide. Most of the 13b polypeptides were S-poor prolamin, whereas 10 kDa, 13a, and 16 kDa polypeptides contained S-rich prolamin in Kinmaze. Thus, it was considered that group A mutants represent S-poor prolamin and group B S-rich prolamin. Isoelectric focusing (IEF) analysis showed that S-poor prolamin level in group A and S-rich prolamin level in group B decreased, but there was no novel band. Because of the simultaneous change in prolamin polypeptides encoded by different structural genes, it was concluded that these mutant genes were involved in regulating the prolamin biosynthesis and/or the accumulation into protein body.
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