Inheritance of cry1A(b) and snowdrop lectin gna genes in transgenic javanica rice progenies and bioassays for resistance to brown planthopper and yellow stem borer
2003
Slamet-Loedin, I.H. | Novalina, S. | Damayanti, D. | Sutrisno | Mulyaningsih, E.S. | Christou, P. | Aswidinoor, H.
Progenies of transgenic lines from Indonesian rice cultivar Rajalele (Karang Agung) containing the cry1A(b) gene and gna were subjected to PCR, Southern, and western analyses and bioassay for yellow stem borer (YSB) and brown planthopper (BPH). Segregation of individual genes in the T1 and T2 generations of positive T0 transgenic lines obtained through particle bombardment was determined using PCR analysis on the plant population that has one or both genes. Results showed that 7 of 12 T1 transgenic lines containing gna and 5 of 7 T1 lines containing cry1A(b) segregated in a Mendelian fashion, whereas, in one transgenic line, genes were not inherited in the second generation. Homozygous plants could be selected in the third generation (T2) in some transgenic lines using PCR analysis. The bioassay for YSB on positive progenies of four T1 transgenic cry1A(b) lines on the stems showed 0 to 100% insect mortality rates, although in all transgenic lines YSB instar growth was suppressed. The bioassay against BPH on the segregated T1 population was scored based on the percentage of plant mortality using BPH type 1. Results showed partial resistance in some lines (plant mortality ranged from 52.5% to 83.5%, compared with 100% of nontransgenic Rajalele and TN-1 sensitive controls. The bioassay on the T2 generation using BPH biotype 2 of Indonesia showed no enhanced resistance in transgenic plants.
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