Evaluation of O. sativa x O. glaberrima - derived lines using microsatellite markers
2003
Ndjiondjop, M.N. | Coburn, J. | Jones, M.P. | McCouch, S.
West African rice breeders have been trying for several decades to develop Oryza sativa varieties that combine high yield potential and stress-adaptation traits (soil-toxicity and drought tolerance, pest and disease resistance). The indigenous African cultivated species, O. glaberrima Steud., is an important reservoir of useful genes for resistance to major stresses, but it has not been much exploited by breeders, mainly because of the lack of fertility of interspecific hybrids. The west Africa Rice Development Association (WARDA) produced several hundred fixed genotypes derived from interspecific crosses between CG14 (O. glaberrima) and japonica rice WAB 56-104 (O. sativa). We selected 50 fixed lines obtained after tissue culture and 50 interbreeding lines. A set of 100 microsatellites dispersed over the 12 chromosomes of rice was used. The polymorphism between the two parents was up to 90%. The regions of the genome carrying O. glaberrima segments will be targeted for the development of near-isogenic lines.
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