Application of transmission electron microscopy and UV-microspectrophotometry for detection of lignin in cell wall of conifers
2004
Gricar, J. | Cufar, K. (Ljubljana Univ. (Slovenia). Biotechnical Fac., Wood Science and Technology Dept.)
Potassium permanganate represents a useful tool in transmission electron microscopy (TEM) for the localization of lignin in various morphological regions of wood. Since this method only provides qualitative estimation, another quantitative method is recommended to verify the results obtained by TEM, such as UV-microspectrophotometry (UMSP). The UMSP method was found to be suited for the determination of lignin content and its distribution in woody tissue. The present paper provides a methodical description and presents the applicability of TEM and UMSP for studies in the topochemistry of lignin distribution within cell walls.
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