Study of in-house reference material preparation for aflatoxin B1 analysis
1996
Duangchan Suprasert
Study of the homogeneity characteristic of aflatoxin B sub(1) (AFB sub(1)) in corn as a reference material was accomplished in this project. Aflatoxin free corn sample was spiked with standard AFB sub(1) solution at the level of 20 parts per billion (ppb) by dry and wet method. They dry method was performed by spiking standard AFB sub(1) solution into dry granulated corn sample using two ways of mixing as arithmetic dilution (AD) and geometric dilution (GD). The wet method was done by spiking standard AFB sub(1) solution into organic solvent suspended corn sample using two kinds of solvent as hexane immersion (HI) and chloroform immersion (CI). Aliquot portions of spiked sample prepared by means of AD, GD, HI and CI were quantitatively AFB sub(1) analysed according to standard operating procedure No. 100602002 which was modified from AOAC official methods of analysis (1990) No. 968.22 "Aflatoxins in peanuts and peanut products". The mean (X) and standard deviation (SD) of AFB sub(1) content in spiked sample from AD, GD, HI and CI were 19.12 and 1.45, 19.22 and 1.23, 18.21 and 1.80 and 19.77 and 0.97, respectively. The result showed that CI was the best way to prepare an in-house reference material for AFB sub(1) analysis, since it gave maximum amount of AFB sub(1) with the smallest deviation.
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